Plant Tissue Culture MCQs Quiz Multiple Choice Questions & Answers

1. What is meant by Organ culture ?

1. Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid
2. Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal
3. Cultivation of organs in a laboratory through the synthesis of tissues
4. The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body

2. What is the name of naturally occurring Auxin in plant?

1. 1-napthaleneacetic acid (NAA)
2. 2,4-Dichlorophenoxyacetic acid (2,4-D)
3. Indole 3-acetic acid (IAA)
4. 1-napthoxyacetic acid (NOA)

3. What is the name of the bacteria known as natural genetic engineer of plants?

1. Escherichia coli
2. Agrobacterium tumefaciens
3. Pseudomonas aeruginosa
4. Aspergillus niger

4. What is/are the benefit(s) of micropropagation or clonal propagation?

1. Rapid multiplication of superior clones
2. Multiplication of disease free plants
3. Multiplication of sexually derived sterile hybrids
4. All of the above

5. When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall?

1. 2-5 days
2. 5-10 days
3. 10-15 days
4. 15-17 days

6. Which breeding method uses a chemical to strip the cell wall of plant cells of two sexually incompatible species?

1. Mass selection
2. Protoplast fusion
3. Transformation
4. Transpiration

7. Which method of plant propagation involves the use of girdling?

1. Grafting
2. Cuttings
3. Layering
4. Micropropagation

8. Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?

1. Multiplication of sexually derived sterile hybrids
2. Less multiplication of disease free plants
3. Storage and transportation of propagates
4. Both b and c

9. Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions?

1. Nurse medium
2. Nurse or feeder culture
3. Both a and b
4. None of these

10. Which of the following plant cell will show totipotency?

1. Xylem vessels
2. Sieve tube
3. Meristem
4. Cork cells

11. Which vector is mostly used in crop improvement?

1. Plasmid
2. Cosmid
3. Phasmid
4. Agrobacterium

12. Who is the father of tissue culture?

1. Bonner
2. Haberlandt
3. Laibach
4. Gautheret

13. A medium which is composed of chemically defined compound is called

1. Natural media
2. Synthetic media
3. Artificial media
4. None of these

14. A(n)...................is an excised piece of leaf or stem tissue used in micropropagation

1. microshoot
2. medium
3. explant
4. scion

15. Agrobacterium based gene transfer is efficient

1. only with dicots
2. only with monocots
3. with both monocots and dicots
4. with majority monocots and few dicots

16. Callus is

1. Tissue that forms embryo
2. An insoluble carbohydrates
3. Tissue that grows to form embryoid
4. Un organized actively dividing mass of cells maintained in cultured

17. Cell fusion method includes the preparation of large number of

1. plant cells stripped of their cell wall
2. single plant cell stripped of their cell wall
3. plant cells with cell wall
4. cells from different species

18. Cellular totipotency is the property of

1. Plants
2. Animals
3. Bacteria
4. all of these

19. Cybrids are produced by

1. Fusion of two different nuclei from two different species
2. Fusion of two same nuclei from same species
3. Nucleus of one species but cytoplasm from both the parent species
4. None of the above

20. DMSO (dimethyl sulfoxide) is used as

1. Gelling agent
2. Alkylating agent
3. Chelating agent
4. Cryoprotectant

21. Growth hormone producing apical dominance is

1. Auxin
2. Gibberellin
3. Ethylene
4. Cytokinin

22. Hormone pair required for a callus to differentiate are

1. Auxin and cytokinin
2. Auxin and ethylene
3. Auxin and absiccic acid
4. Cytokinins and gibberllin

23. In a callus culture

1. increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
2. increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
3. auxins and cytokinins are not required
4. only auxin is required for root and shoot formation

24. In a cell, protoplast consists of the following EXCEPT?

1. Cell wall
2. Cell membrane
3. Nucleus
4. Cytoplasm

25. In growth room, humidifier serves as?

1. Contaminant reducer
2. Humidity reducer
3. Medium drying preventer
4. Temperature controller

26. In plant tissue culture, what is the term ORGANOGENESIS means?

1. Formation of callus culture
2. Formation of root & shoot from callus culture
3. Genesis of organ
4. None of the above

27. Media room of a plant tissue culture lab should consist of the following EXCEPT?

1. pH meter
2. Autoclave machine
3. Analytical balance
4. Biosafety cabinet

28. Organogenesis is

1. formation of callus tissue
2. formation of root and shoots on callus tissue
3. Both a and b
4. genesis of organs

29. Part of plant used for culturing is called

1. Scion
2. Explant
3. Stock
4. Callus

30. Plant tissue culture technique is a redefined method of

1. Hybridization
2. Vegetative Propagation
3. Asexual Reproduction
4. Selection

31. Polyethylene glycol is

1. Fusogenic chemical
2. Electrofusion stimulant
3. Callus stimulant
4. Differentiation stimulant

32. Protoplast are the cells devoid of

1. cell membrane
2. cell wall
3. both cell wall and cell membrane
4. none of these

33. Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with

1. cellulotyic enzymes
2. pectolytic enzymes
3. both cellulotyic and pectolytic enzymes
4. proteolytic enzymes

34. Somaclonal variations are the ones

1. Caused by mutagens
2. Produce during tissue culture
3. Caused by gamma rays
4. Induced during sexual embryogeny

35. Somatic hybridization is achieved through

1. Grafting
2. Protoplast fusion
3. Conjugation
4. Recombinant DNA technology

36. Subculturing is similar to propagation by cuttings because

1. it separates multiple microshoots and places them in a medium
2. it uses scions to produce new microshoots
3. they both use in vitro growing conditions
4. all of the abov

37. Synthetic seed is produced by encapsulating somatic embryo with

1. Sodium Chloride
2. Sodium alginate
3. Sodium acetate
4. Sodium nitrate

38. The ability of the component cells of callus to form a whole plant is known as

1. Redifferentiation
2. Dedifferentiation
3. either a or b
4. none of these

39. The enzymes required to obtain wall-free / naked protoplasts are

1. Cellulase and Proteinase
2. Cellulase and Pectinase
3. Cellulase and amylase
4. Amylase and Pectinase

40. The first transgenic crop was

1. Pea
2. Tobacco
3. Flax
4. Cotton

41. The most widely used chemical for protoplast fusion, as fusogen is

1. Manitol
2. Sorbitol
3. Mannol
4. Polyethylene glycol

42. The phenomenon of the reversion of mature cells to the meristematic state leading to the formation of callus is known as

1. Redifferentiation
2. Dedifferentiation
3. either a or b
4. none of these

43. The production of secondary metabolites require the use of

1. Protoplast
2. Cell suspension
3. Meristem
4. Auxillary buds

44. To obtain haploid plant, we culture

1. Entire anther
2. Nucleus
3. Embryo
4. Apical bud