Plant Tissue Culture Multiple Choice Questions and Answers

1. What is meant by Organ culture ?

1. Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid
2. Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal
3. Cultivation of organs in a laboratory through the synthesis of tissues
4. The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body

2. What is the name of naturally occurring Auxin in plant?

1. 1-napthaleneacetic acid (NAA)
2. 2,4-Dichlorophenoxyacetic acid (2,4-D)
3. Indole 3-acetic acid (IAA)
4. 1-napthoxyacetic acid (NOA)

3. What is the name of the bacteria known as natural genetic engineer of plants?

1. Escherichia coli
2. Agrobacterium tumefaciens
3. Pseudomonas aeruginosa
4. Aspergillus niger

4. What is/are the benefit(s) of micropropagation or clonal propagation?

1. Rapid multiplication of superior clones
2. Multiplication of disease free plants
3. Multiplication of sexually derived sterile hybrids
4. All of the above

5. When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall?

1. 2-5 days
2. 5-10 days
3. 10-15 days
4. 15-17 days

6. Which breeding method uses a chemical to strip the cell wall of plant cells of two sexually incompatible species?

1. Mass selection
2. Protoplast fusion
3. Transformation
4. Transpiration

7. Which method of plant propagation involves the use of girdling?

1. Grafting
2. Cuttings
3. Layering
4. Micropropagation

8. Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?

1. Multiplication of sexually derived sterile hybrids
2. Less multiplication of disease free plants
3. Storage and transportation of propagates
4. Both b and c

9. Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions?

1. Nurse medium
2. Nurse or feeder culture
3. Both a and b
4. None of these

10. Which of the following plant cell will show totipotency?

1. Xylem vessels
2. Sieve tube
3. Meristem
4. Cork cells