Plant Tissue Culture MCQ Questions and Answers for Practice

1. What is meant by Organ culture ?

1. The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body
2. Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid
3. Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal
4. Cultivation of organs in a laboratory through the synthesis of tissues

2. What is the name of naturally occurring Auxin in plant?

1. 1-napthoxyacetic acid (NOA)
2. 1-napthaleneacetic acid (NAA)
3. 2,4-Dichlorophenoxyacetic acid (2,4-D)
4. Indole 3-acetic acid (IAA)

3. What is the name of the bacteria known as natural genetic engineer of plants?

1. Aspergillus niger
2. Escherichia coli
3. Agrobacterium tumefaciens
4. Pseudomonas aeruginosa

4. What is/are the benefit(s) of micropropagation or clonal propagation?

1. All of the above
2. Rapid multiplication of superior clones
3. Multiplication of disease free plants
4. Multiplication of sexually derived sterile hybrids

5. When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall?

1. 15-17 days
2. 2-5 days
3. 5-10 days
4. 10-15 days

6. Which breeding method uses a chemical to strip the cell wall of plant cells of two sexually incompatible species?

1. Transpiration
2. Mass selection
3. Protoplast fusion
4. Transformation

7. Which method of plant propagation involves the use of girdling?

1. Micropropagation
2. Grafting
3. Cuttings
4. Layering

8. Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?

1. Both b and c
2. Multiplication of sexually derived sterile hybrids
3. Less multiplication of disease free plants
4. Storage and transportation of propagates

9. Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions?

1. None of these
2. Nurse medium
3. Nurse or feeder culture
4. Both a and b

10. Which of the following plant cell will show totipotency?

1. Cork cells
2. Xylem vessels
3. Sieve tube
4. Meristem

11. Which vector is mostly used in crop improvement?

1. Agrobacterium
2. Plasmid
3. Cosmid
4. Phasmid

12. Who is the father of tissue culture?

1. Gautheret
2. Bonner
3. Haberlandt
4. Laibach

13. A medium which is composed of chemically defined compound is called

1. None of these
2. Natural media
3. Synthetic media
4. Artificial media

14. A(n)...................is an excised piece of leaf or stem tissue used in micropropagation

1. scion
2. microshoot
3. medium
4. explant

15. Agrobacterium based gene transfer is efficient

1. with majority monocots and few dicots
2. only with dicots
3. only with monocots
4. with both monocots and dicots