Correct Answer is: Un organized actively dividing mass of cells maintained in cultured

Correct Answer is: formation of root and shoots on callus tissue

Un organized actively dividing mass of cells maintained in cultured

Tissue that grows to form embryoid

increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation

increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation

auxins and cytokinins are not required

only auxin is required for root and shoot formation

formation of root and shoots on callus tissue

Plant Tissue Culture MCQ Multiple Choice Questions Answers for Practice

Q: 1. A medium which is composed of chemically defined compound is called

Correct Answer is: Synthetic media

Q: 3. Agrobacterium based gene transfer is efficient

Correct Answer is: only with dicots

Q: 5. Cell fusion method includes the preparation of large number of

Correct Answer is: single plant cell stripped of their cell wall

Q: 7. Cybrids are produced by

Correct Answer is: Nucleus of one species but cytoplasm from both the parent species

Q: 8. DMSO (dimethyl sulfoxide) is used as

Correct Answer is: Cryoprotectant

Q: 10. Hormone pair required for a callus to differentiate are

Correct Answer is: Auxin and cytokinin

Q: 11. In a callus culture

Correct Answer is: increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation

Q: 13. In growth room, humidifier serves as?

Correct Answer is: Medium drying preventer

Q: 14. In plant tissue culture, what is the term ORGANOGENESIS means?

Correct Answer is: Formation of root & shoot from callus culture

1. A medium which is composed of chemically defined compound is called

Natural media
Synthetic media
Artificial media
None of these

2. A(n)...................is an excised piece of leaf or stem tissue used in micropropagation

microshoot
medium
explant
scion

3. Agrobacterium based gene transfer is efficient

only with dicots
only with monocots
with both monocots and dicots
with majority monocots and few dicots

4. Callus is

Tissue that forms embryo
An insoluble carbohydrates
Tissue that grows to form embryoid
Un organized actively dividing mass of cells maintained in cultured

5. Cell fusion method includes the preparation of large number of

plant cells stripped of their cell wall
single plant cell stripped of their cell wall
plant cells with cell wall
cells from different species

6. Cellular totipotency is the property of

Plants
Animals
Bacteria
all of these

7. Cybrids are produced by

Fusion of two different nuclei from two different species
Fusion of two same nuclei from same species
Nucleus of one species but cytoplasm from both the parent species
None of the above

8. DMSO (dimethyl sulfoxide) is used as

Gelling agent
Alkylating agent
Chelating agent
Cryoprotectant

9. Growth hormone producing apical dominance is

Auxin
Gibberellin
Ethylene
Cytokinin

10. Hormone pair required for a callus to differentiate are

Auxin and cytokinin
Auxin and ethylene
Auxin and absiccic acid
Cytokinins and gibberllin

11. In a callus culture

increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
auxins and cytokinins are not required
only auxin is required for root and shoot formation

12. In a cell, protoplast consists of the following EXCEPT?

Cell wall
Cell membrane
Nucleus
Cytoplasm

13. In growth room, humidifier serves as?

Contaminant reducer
Humidity reducer
Medium drying preventer
Temperature controller

14. In plant tissue culture, what is the term ORGANOGENESIS means?

Formation of callus culture
Formation of root & shoot from callus culture
Genesis of organ
None of the above

15. Media room of a plant tissue culture lab should consist of the following EXCEPT?

pH meter
Autoclave machine
Analytical balance
Biosafety cabinet

16. Organogenesis is

formation of callus tissue
formation of root and shoots on callus tissue
Both a and b
genesis of organs

17. Part of plant used for culturing is called

Scion
Explant
Stock
Callus

18. Plant tissue culture technique is a redefined method of

Hybridization
Vegetative Propagation
Asexual Reproduction
Selection

19. Polyethylene glycol is

Fusogenic chemical
Electrofusion stimulant
Callus stimulant
Differentiation stimulant

20. Protoplast are the cells devoid of

cell membrane
cell wall
both cell wall and cell membrane
none of these

21. Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with

cellulotyic enzymes
pectolytic enzymes
both cellulotyic and pectolytic enzymes
proteolytic enzymes

22. Somaclonal variations are the ones

Caused by mutagens
Produce during tissue culture
Caused by gamma rays
Induced during sexual embryogeny

23. Somatic hybridization is achieved through

Grafting
Protoplast fusion
Conjugation
Recombinant DNA technology

24. Subculturing is similar to propagation by cuttings because

it separates multiple microshoots and places them in a medium
it uses scions to produce new microshoots
they both use in vitro growing conditions
all of the abov

25. Synthetic seed is produced by encapsulating somatic embryo with

Sodium Chloride
Sodium alginate
Sodium acetate
Sodium nitrate

26. The ability of the component cells of callus to form a whole plant is known as

Redifferentiation
Dedifferentiation
either a or b
none of these

27. The enzymes required to obtain wall-free / naked protoplasts are

Cellulase and Proteinase
Cellulase and Pectinase
Cellulase and amylase
Amylase and Pectinase

28. The first transgenic crop was

Pea
Tobacco
Flax
Cotton

29. The most widely used chemical for protoplast fusion, as fusogen is

Manitol
Sorbitol
Mannol
Polyethylene glycol

30. The phenomenon of the reversion of mature cells to the meristematic state leading to the formation of callus is known as

Redifferentiation
Dedifferentiation
either a or b
none of these

Plant Tissue Culture MCQs are essential for students and exam aspirants to test their knowledge and understanding of the subject. Practice these multiple-choice questions to excel in your exams and interviews.

About Plant Tissue Culture MCQ Questions

Plant Tissue Culture is a method of growing plant cells, tissues, or organs under aseptic conditions. Key concepts covered include the types of cultures (e.g., callus, suspension), media components, and applications in agriculture and biotechnology. These MCQs will help you grasp the fundamental and advanced aspects of plant tissue culture.

Why Practice Plant Tissue Culture Objective Questions?

Practicing Plant Tissue Culture MCQs offers several benefits. It helps in reinforcing your understanding of key concepts, preparing for school and college exams, and enhancing your performance in competitive exams and interviews. Regular practice also aids in identifying weak areas and improving your overall subject knowledge.

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