Plant Tissue Culture Multiple Choice Questions and Answers

21. Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with

1. cellulotyic enzymes
2. pectolytic enzymes
3. both cellulotyic and pectolytic enzymes
4. proteolytic enzymes

22. Somaclonal variations are the ones

1. Caused by mutagens
2. Produce during tissue culture
3. Caused by gamma rays
4. Induced during sexual embryogeny

23. Somatic hybridization is achieved through

1. Grafting
2. Protoplast fusion
3. Conjugation
4. Recombinant DNA technology

24. Subculturing is similar to propagation by cuttings because

1. it separates multiple microshoots and places them in a medium
2. it uses scions to produce new microshoots
3. they both use in vitro growing conditions
4. all of the abov

25. Synthetic seed is produced by encapsulating somatic embryo with

1. Sodium Chloride
2. Sodium alginate
3. Sodium acetate
4. Sodium nitrate

26. The ability of the component cells of callus to form a whole plant is known as

1. Redifferentiation
2. Dedifferentiation
3. either a or b
4. none of these

27. The enzymes required to obtain wall-free / naked protoplasts are

1. Cellulase and Proteinase
2. Cellulase and Pectinase
3. Cellulase and amylase
4. Amylase and Pectinase

28. The first transgenic crop was

1. Pea
2. Tobacco
3. Flax
4. Cotton

29. The most widely used chemical for protoplast fusion, as fusogen is

1. Manitol
2. Sorbitol
3. Mannol
4. Polyethylene glycol

30. The phenomenon of the reversion of mature cells to the meristematic state leading to the formation of callus is known as

1. Redifferentiation
2. Dedifferentiation
3. either a or b
4. none of these